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Contact

Ms. Sarah Bianco, Manager
Phone: 919.513.6443
Email: seschuet@ncsu.edu

Instrumentation

Policies

Flow Cytometry and Cell Sorting Laboratory

 

Analyzers

Becton Dickinson LSRII

Has three lasers, 488 nm, 635 nm and 405 nm. Can analyze up to nine colors and utilizes BD Diva software for acquisition. Scheduling for the LSRII is available by online invitation calendar. Click here to access the LSRII configuration for experiment planning.

BD LSRII

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Becton-Dickinson FACSCaliburFACSCalibur

Provides all of the same capabilities as the FACScan. In addition it has a second excitation laser (633nm) and, thus, is capable of analyzing up to four colors of fluorescence. The 4th flourochrome used is usually allophycocyanin (APC).

All analyzers have the capacity to do DNA analysis using Propidium Iodide or other stains excited by a 488 nm laser or 633 nm laser.

 

 

Cytek modified BD FACScanCytek modified BD FACScan

Has been modified with a 635 nm laser and two additional PMTS to provide a total of five colors from the 488 nm and 635 nm lasers. Utilizes FlowJo Collection Software for acquisition and FlowJo for analysis.