Flow Cytometry and Cell Sorting Laboratory
Analyzers
Becton-Dickinson FASCan
Has a single argon laser with an excitation wavelength of 488nm. It can provide data for forward scatter (FSC), side scatter (SSC), and 3 colors of fluorescence. The fluorescence wavelengths that can be routinely collected are FL1 (525nm), FL2 (575nm), and FL3 (>650nm). The fluorochromes that are best suited for routine use on the FACScan are:
- FL1: fluorescein, GFP (green fluorescent protein) or some of the fluorescein alternatives such as Alexa 488
- FL2: R-phycoerythrin (R-PE)(Cy3 can also be detected)
- FL3: PerCP or R-PE-Cy5
Becton-Dickinson FACSCalibur
Provides all of the same capabilities as the FACScan. In addition is has a second excitation laser (633nm) and, thus, is capable of analyzing up to 4 colors of fluorescence. The 4th flourochrome used is usually allophycocyanin (APC).
Both analyzers have the capacity to do DNA analysis using Propidium Iodide or other stains excited by a 488 nm laser or 633 nm laser.