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Leukocyte Biology Lab

Welcome to the Leukocyte Biology Laboratory web site.This site is designed to provide information about us, our research, and our collaborative investigations. It also contains links to many sites of interest.

Lab Members

Sam Jones, Principal Investigator

Research

The Leukocyte Biology Laboratory is interested in understanding the molecular mechanisms of phagocyte activation. Specifically, we are studying the role of cytoskeletal proteins such as the leukocyte specific actin bundling protein L-plastin (LPL), in regulating the signaling cascades which lead to the development of the effector phenotype in neutrophils and macrophages. We are defining the role of LPL and the actin cytoskeleton in regulating signal transduction pathways during integrin activation and the role of beta 2 integrin (CD11/CD18 complex) activation in adhesion, respiratory burst and reactive oxygen production, degranulation, prostaglandin and leukotriene production, migration, and phagocytosis.

We are applying this information to clinically important inflammatory diseases, particularly gastrointestinal inflammation and arthritis. For example, we are studying the role of neutrophils in mucosal injury during gastrointestinal inflammation induced by ischemic injury and the mechanism of neutrophil and macrophage activation in immune complex disease such as rheumatoid arthritis. We are also interested in the strategies used by intracellular bacterial gastrointestinal pathogens such as Salmonella, to invade macrophages, modulate their activation, induce apoptosis, and subsequently evade the host immune response.

Our laboratory collaborates extensively with Anthony Blikslager and the Gastrointestinal Physiology Laboratory, and the Center for Gastrointestinal Biology and Disease at UNC-Chapel Hill.

Selected Publications

For an updated Medline search click on Sam Jones Publications

Jones SL, Blikslager AT. (2002) Role of the enteric nervous system in the pathophysiology of secretory diarrhea. J Vet Intern Med 16:222-228.

Campbell NB, Jones SL, Blikslager AT. (2002) The effects of cyclooxygenase inhibitors on bile-injured and normal equine colon. Equine Vet J 34:493-498.

Gayle J, Jones SL, Argenzio RA, Blikslager AT. (2002) Neutrophils increase paracellular permeability of restituted ischemic injured porcine ileum. Surgery (In Press).

Chilcoat CD, Rowlingson KA, Jones SL. (2002) The effects of cAMP modulation upon the adhesion and respiratory burst activity of immune complex-stimulated equine neutrophils. Vet Immunol Immunopathol 88:65-77.

Jones SL. (2002) Protein kinase A regulates ?2 integrin avidity in neutrophils. J Leukoc Biol 71:1042-1048.

Jones SL, Sharief Y, Chilcoat CD. (2001) Signaling mechanism for equine neutrophil activation by immune complexes. Vet Immunol Immunopath 82:87-100.

Jones SL, Blikslager AT. (2001) Future directions in anti-inflammatory therapy. Vet Clin North Am Equine Pract 17:245-262.

Gayle JM, Blikslager AT, Jones SL. (2000) Role of neutrophils in intestinal mucosal injury. J Am Vet Med Assoc 217:498-500.

Jones SL, Wang J, Turck CW, Brown EJ. (1998) A role for the actin-bundling protein L- plastin in the regulation of leukocyte integrin function. Proc Natl Acad Sci,USA. 95:9331-9336.

Jones SL, Knaus UG, Bokoch GM, Brown EJ. (1998) Two signaling mechanisms for activation of ?M ?2 avidity in polymorphonuclear neutrophils. J Biol Chem. 273:10556-10566.

Jones SL, Brown EJ. (1996) Fc?RII-mediated adhesion and phagocytosis induce L-plastin phosphorylation in human neutrophils. J Biol Chem. 271:14623-14630.

Rosales C, Jones SL, McCourt D, Brown EJ. (1994) Bromophenacyl bromide binding to the actin-bundling protein l-plastin inhibits inositol triphosphate-independent increase in Ca2+ in human neutrophils. Proc Natl Acad Sci USA. 91:3534-3538.

Jones SL, Lindberg FL, Brown EJ. (1999) Phagocytosis. In: Paul WE (ed). Fundamental Immunology. Raven Press, New York, NY. pp 997-1020.

Jones SL, Brown EJ. Functional cooperation between Fc receptors and complement receptors in phagocytes. In: van de Winkel JGJ, Capel PJA (eds). Human IgG Fc Receptors RG Landes Co, Georgetown, Texas, 1996.

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