Hammerberg, Bruce DVM PhD
Professor

DVM: Michigan State University
PhD: Michigan State University
M.S.: Michigan State University

Phone: 919-513-7712
Fax: 919-515-4237
Email: Bruce_Hammerberg@ncsu.edu

Research Area
Allergic diseases and nematode biology

Current Research
My current research expertise is in allergic diseases and nematode biology. Regarding allergic disease research I have capitalized on my recent development of canine x murine heterohybridomas. One of these produces canine monoclonal IgE specific for a filarial nematode antigen. Another produces canine IgG antibodies specific for canine IgE. Using these tools I have developed mouse monoclonal antibodies against heat stable epitopes of canine IgE and have the opportunity to make canine monoclonal antibodies against canine IgE epitopes that will be useful in preventing allergic disease in the dog. The unlimited supply of canine IgE of known antigen specificity has directed my research toward characterizing inherited differences in mast cell function in thedog, and at this time I am investigating the role of stem cell factor in inherited canine atopic dermatitis.

Most Recently I have developed a monoclonal antibody based ELISA for measuring allergin-specific and total IgE in horse serum.

My work with filarial nematodes over the last 25 years has recently turned to the discovery of parasite products having anti-inflammatory properties through inhibition of mast cell activation and degranulation.

Current Collaborators
Drs. Hilary Jackson and Thierry Olivry in the Center for Comparative Molecular Medicine.

Future Research
Development of anti-IgE vaccines for the immunotherapy of canine allergic diseases and as a model for efficacy and safety of anti-IgE vaccines for human allergic diseases.

Identification of heritable traits affecting mast cell function in allergic diseases using the canine model.

Characterization of mast cell receptors responsible for release of tumor necrosis factor-alpha in dogs and horses.

Publications

Tater, C, Jackson, HA, Paps, J, Hammerberg, B. 2005. Routine prophylactic vaccination enhances an allergen specific serum IgE and IgG response in allergic dogs but this response is not seen with aluminum adjuvant alone. American Journal of Veterinary Research in press.

Hammerberg, B. Chapter 4: Canine Immune System. In: Animal Models of Human Inflammatory Skin Diseases. Pp. 79-90. Ed. Lawrence S. Chan. CRC Press, Boca Raton, Fl. 2004.

Jackson, H.A., Jackson, M.W., Coblentz, L., Hammerberg, B. 2003. Evaluation of the clinical and allergen specific serum IgE response to oral challenge with cornstarch, corn, soy and a soy hydrolysate in dogs with spontaneous food allergy. Vet. Derm. 14:181-187.

Little, D., Flowers, J.R., Hammerberg, B., Gardner, S.Y. 2003. Management of drug-resistant cyathostominosis on a breeding farm in central North Carolina. Equine Veterinary Journal 35:246-251.

Kimber, I., Dearman, R.J., Penninks, A.H., Knippels, L.M.J., Buchanan, R.B., Hammerberg, B., Jackson, H.A. and Helm, R.M. 2003. Assessment of protein allergenicity on the basis of immune reactivity: animal models. Environmental Health Perspectives 111:1125-1130.

Wang, C.Y., Walfield, A.M., Fang, X., Hammerberg, B., Ming, J.Y., Li, L., Shen, M., Alexander, V., MacGlashan, D.W. 2003. Synthetic IgE peptide vaccine for immunotherapy of allergy. Vaccine 21:1580-1590.

Jackson, H. A., Smith, C., Hammerberg, B. 2002. Evaluation of a spontaneous canine model of IgE mediated food hypersensitivity: dynamic changes in serum, faecal and allergen-specific IgE relative to dietary change. Comp. Med. 52:316-321.

Watson BI, Hammerberg B.. Identification of a collectin-llike protein in pig serum that binds a component in perienteric fluid from Ascaris suum. Comparative Immunology, Microbiology and Infectious Disease.. 23: 113-124, 2000

Vaden SL, Hammerberg B, Davenport DJ, Orton SM, Trogdon MM, Melgarejo T, Van Camp SD, Williams DA.SD. Food hypersensitivity reaction in soft coated wheaten terriers with protein-losing enteropathy or protein-losing nephropathy or both: gastroscopic food sensitivity testing, dietary provocation and fecal immunoglobulin E. Journal of Veterinary Internal Medicine. 14: 60-67, 2000

Ediriwickrema CS, Tonkonogy S, Hammerberg B.. Natural killer cell-dependent immunoglobulin G2a anti-bovine serum albumin (BSA) reponse elicited by high molecular weight dextran-BSA conjugates associated with dextran-mediated macrophage-NK cell interaction. Immunology. 101: 474-483, 2000

Orton SM, Weinstock D, Hammerberg B.. Association of elevated lymph node cell release of histamine and tumor necrosis factor with genetic predisposition to limb edema formation in dogs infected with Brugia pahangi. American Journal of Tropical Medicine and Hygiene. 58: 695-704, 1998

Hammerberg B, Bevier D, DeBoer DJ, Olivry T, Orton SM, Gebhard D, Vaden SL. Auto IgG anti-IgE and IgG x IgE immune complex presence and effects on ELISA-based quantitation of IgE in canine atopic dermatitis, demodectic acariasis and helminthiasis. Veterinary Immunology and Immunopathology. 60: 33-46, 1997

Schreuer D. and Hammerberg B. Inhibition by platelets of invitro blastogenic responses of canine PBMC by a PAF-dependent mechanism. Vet. Immunol. and Immunopathol., 52:135-145, 1996

Gebhard D., Orton S., Edmiston D., Nakagaki K., DeBoer D. and Hammerberg B. Canine IgE monoclonal antibody specific for a filarial antigen: production by a canine X murine heterohybridoma using B cells from a clinically affected lymph node. Immunology 85:429-434, 1995

Schreuer D. and Hammerberg B. Modulation of cellular and humoral immunity, and disease manifestation during onset of patency in Brugia pahangi-infected dogs. Immunology 79:658-666, 1993

Miller S., Schreuer D. and Hammerberg B. Inhibition of antigen-driven proliferative responses and enhancement of antibody production during infection with Brugia pahangi. J. Immunol. 147:1007-1013, 1991

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