Talmage Brown, DVM, PhD
DVM: Oklahoma State University
PhD: Cornell University
Post Doctoral: The Johns Hopkins
University School of
Diplomate of American College of Veterinary Pathologists
2002-Present Professor, Department of Farm Animal Health and Resource Management, College of Veterinary Medicine, North Carolina State University.
1981-2002 Professor, Department of Microbiology, Pathology, and Parasitology,
College of Veterinary Medicine, North Carolina State University.
1979-1981 Research Leader, Shipping Fever and Respiratory Diseases of Cattle
Project, USDA, National Animal Disease Center, Ames, IA.
1978-1979 Acting Research Leader, Reproductive Diseases of Swine Project, USDA, National Animal Disease Center, Ames, IA.
1975-1978 Assistant Professor, Oklahoma State University.
1974-1975 Assistant Professor, The Johns Hopkins University School of Medicine.
1973-1974 Postdoctoral Fellow, The Johns Hopkins University School of Medicine.
1972-1973 Research Associate, New York State Veterinary College.
1968-1972 Graduate Research Assistant, New York State Veterinary College.
1967-1968 Small Animal Practice, Durham, North Carolina.
1965-1967 Base Veterinarian, U. S. Air Force, Karamursel Air Station, Turkey.
1. Gene therapy using a dog model of glycogen storage disease type Ia
GSD Ia is an inherited metabolic disorder caused by glucose-6-phosphatase (G6Pase) deficiency, and we have characterized a canine model for GSD Ia. Currently, nutritional treatment has improved outcome; however, hypoglycemia and long-term complicationsstill occur. Comnplete biochemical correction of GSD Ia is possible by replacement of G6Pase in liver, as shown by liver transplantation. We have demonstrated G6Pase introduction in the GSD Ia liver following administration of an AAV vector, accompanied by decreased liver glycogen content and correction of hypoglycemia and hypercholesterolemia. We are now pursuing regulated expression of G6Pase driven by the endogenous G6Pase promoter. Regulated G6Pase expression with AAV vectors could promote normal glucose metabolism and prevent long-term complications of GSD Ia in the canine model.
- Dr. Jody Gookin
- Dr. Dwight Koeberl (Duke University Medical Center)
- Dr. Melanie Rembert
- Dr. Mike Whitacre
RM Beaty, M Jackson, D Peterson, A Bird, T Brown, DK Benjamin, T. Juoperri, P Kishnani, A Boney, Y-T Chen, D Koeberl. Delivery of glucose-6-phosphatase in a canine model for glycogen storage disease, type Ia with Adeno-associated virus (AAV). Gene Therapy. 9:1015-1022, 2002.
BL Pappalardo, TT Brown, MB Tompkins, EB Breitschwerdt. Immunopathology of Bartonella vinsonii (berkhoffi) in experimentally infected dogs. Veterinary Immunology and Immunopathology. 83: 125-147, 2001.
W-H Feng, MB Tompkins, J-S Xu, TT Brown, SM Laster, H Zhang, MB McCaw. Thymocyte and peripheral blood T lymphocyte subpopulation changes in piglets following in utero infection with porcine reproductive and respiratory syndrome virus. Journal of Virology. 75: 4889-4895, 2001.
PS Kishnani, E Faulkner, S VanCamp, M Jackson, T Brown, A Boney, D Koeberl, Y-T Chen. Canine model and genomic structural organization of glycogen storage disease Type Ia (GSD Ia). Veterinary Pathology. 38: 83-91, 2001.
BL Pappalardo, T Brown, D Gebhardt, S Sontakke, EB Breitschwerdt. Cyclic CD8+ lymphopenia in dogs experimentally infected with Bartonella vinsonii subsp. Bekhoffii. Veterinary Immunology and Imunopathology. 75: 43-57, 2000.
SC Henry, K Schmader, TT Brown, SE Miller, DN Howell, GG Daley, JD Hamilton. Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection. Journal of Virological Methods. 89: 61-73, 2000.
Staff: Kwang Ok Shin