Breen, Matthew, PhD. C.Biol. M.I.Biol
Professor of Genomics

E-mail: matthew_breen@ncsu.edu

ONGOING RECRUITMENT OF CANINE TUMORS

For further details on recruitment please contact CVM_K9Genomics@ncsu.edu

Recruitment information – Canine Soft Tissue Sarcomas, Histiocytic Malignancies and Hemangiosarcoma

Recruitment Information - Canine lymphoma and leukemia
We are recruiting cases of canine lymphoma and leukemia and ask that you contact CVM_K9Genomics@ncsu.edu for further information on how you may participate.

Research Focus:

• Overview
• Canine karyotype
• Molecular Cytogenetics
• Array-CGH analysis of canine tumors
• Gene Expression Analysis
• Canine Brain Tumor Research
• Integrated Genome Mapping

Biography:

Dr. Matthew Breen graduated with honors in Genetics from the University of Liverpool, U.K. in 1987. He completed his PhD, working on cytogenetics of the Equidae in 1990. Dr. Breen was employed as a Post Doctoral research scientist in Molecular Genetics at the U.K. Medical Research Council's Human Genetics Unit in Edinburgh, Scotland, where he was responsible for developing improved fluorescence in situ hybridization techniques as part of the Human Genome Mapping Project. Dr. Breen then spent four years working for the Australian Thoroughbred industry, based at the University of Queensland, Brisbane Australia. In 1996 Dr. Breen returned to the U.K. where his laboratory developed molecular cytogenetics reagents, resources and techniques for application to canine and equine genome mapping, comparative cytogenetics and cancer cancer studies. In 1998 Dr. Breen was awarded membership of the Institute of Biology and the title of Chartered Biologist.

In 2002 Dr. Breen relocated his laboratory to NCSU's College of Veterinary Medicine as part of their Genomics initiative. His research interests continue to focus on genomics, genome mapping and the comparative aspects of canine cancer.

Laboratory Personnel:

Keara Boss - Clinician Scientist
mboss@ncsu.edu

Kate Byler BS - Research Assistant
kate_byler@ncsu.edu

Anita Croasmun BS - Research Assistant
anita_croasmun@ncsu.edu

Shannon Duke BSc - CBS Graduate Student
Shannon_Duke@ncsu.edu

Benoit Hedan PhD - Post Doc
benoit_hedan@ncsu.edu

Stephanie Montogomery, PhD - Post Doc/ DVM student
samontgo@ncsu.edu

Tsai Pei-Chien - CBS Graduate Student
ptsai@unity.ncsu.edu

Patrick Proctor - Student
paprocto@ncsu.edu

Katie Saylor – Student
kdsaylor@ncsu.edu

Eric Seiser – Functional Genomics Graduate Student
elseiser@ncsu.edu

Rachael Thomas, PhD C.Biol., M.I.Biol - Research Assistant Professor
Rachael_Thomas@ncsu.edu

Christina Williams – MSc – Research Assistant
Christina_Williams@ncsu.edu

Andrea Young – Functional Genomics Graduate Student
acyoung@ncsu.edu

Selected Publications:

Breen, M. Canine cytogenetics - from band to basepair
Cytogenet Genome Res 2008;120:50-60

Breen. M and Langford, C.F. (2008).  Chromosome Structure and Function - Comparative Cytogenetics. In: Encyclopedia of the Human Genome. Macmillan Publishers Ltd. Nature Publishing Group, Hampshire, U.K.

Breen, M. and Modiano, J. (2008). Evolutionarily Conserved Cytogenetic Changes in Hematologic Malignancies of Dogs and Humans – Man and his best friend share more than companionship. Chromosome Research 16:145–154. 

Thomas, R., Duke, S. Bloom, S., Breen, T,Feiste, E., Young, A., Seiser, E., Tsai, Pei Chien, Ellis, P., Langford, C, Karlsson, E., Mauceli, E., Lindblad-Toh, K., Breen. M. (2007). A cytogenetically characterized, genome anchored 10Mb BAC array for the domestic dog. J. Heredity  98 (5): 474-484.

Fosmire SP, Thomas R, Jubala CM, Smith T, Wojcieszyn J, Getzy DM, Valli VEO, Helfand SC, Cutter GC, Breen M¤, Modiano JF¤. (2007). Inactivation of p16 cyclin-dependent kinase inhibitor in high-grade canine non-Hodgkin T cell lymphoma. Vet Path. 44(4): 467-478
¤ co-senior authors

Modiano JF, Breen M, Valli VEO, Wojcieszyn, J.W, Cutter, G. (2007).  Predictive Value of p16 or Rb Inactivation in a Model of Naturally Occurring Canine non-Hodgkin Lymphoma. Leukemia 21(1): 184-187.

Tarjei S. Mikkelsen, Matthew J. Wakefield, Bronwen Aken, Chris T. Amemiya, Jean L. Chang, Shannon Duke (..and others),  Matthew Breen, Paul B. Samollow, Eric S. Lander & Kerstin Lindblad-Toh (2007). Genome of the marsupial Monodelphis domestica reveals innovation in non-coding sequences. Nature 447, 167-177

Duke, S., Samallow, P., Mauceli, E., Lindblad-Toh, K., Breen, M. (2007). Cytogenetic BAC map of the genome of Monodelphis domestica.Chromosome Research 15:3610-370
(Companion paper to the Monodelphis genome paper)

Davidow, L.S., Breen, M., Samollow, P.B., Duke,  S.E., McCarrey, J.R. and Lee, J.T. (2007).  The Search for a Marsupial XIC Reveals a Break with Vertebrate Synteny. Chromosome Research 15:137-146.
(Companion paper to the Monodelphis genome paper)

Khanna, C. Lindblad-Toh, K., Vail, D. London, C. Bergamn, P. Barber, L. Breen, M., Kitchell, B., McNeil, E., Modiano, J.F., Niemi, S., Comstack, K.E., Ostrander, E., Westmoreland, S., Withrow S. 2006. The dog as a cancer model. Nature Biotech. (Letter to the Editor) 24:1065-6.

Modiano, J.F. and Breen, M. Genetic Basis of Cancer. In Clinical Oncology, Elsevier. (in press)

Modiano JF, Breen M, Lana SE, Ehrhart EJ, Schaack J, Duke RC, Cutter GC, Bellgrau D (2006). Naturally occurring translational models for development of cancer gene therapy. Gene Therapy and Molecular Biology 10, 31-40, 2006

Breen, M. and Thomas, R. Karyotype and Chromosomal Organization. In The Dog and its Genome. Ostrander EA, Giger U, Lindblad-Toh K, eds. Cold Spring Harbor Press:Cold Spring Harbor, 2005.

Modiano JF, Breen M, London CA, Avery AC. Breed Specific Canine Lymphoproliferative Diseases. In The Dog and its Genome. Ostrander EA, Giger U, Lindblad-Toh K, eds. Cold Spring Harbor Press:Cold Spring Harbor, 2005.

Rachael Thomas, Allyson Scott, Cordelia F. Langford, Susan P. Fosmire, Cristan M. Jubala, Travis D. Lorentzen, Christophe Hitte, Elinor K. Karlsson, Ewen Kirkness, Elaine A. Ostrander, Francis Galibert, Kerstin Lindblad-Toh, Jaime F. Modiano, and Matthew Breen. Construction of a 2-Mb resolution BAC microarray for CGH analysis of canine tumors. Genome Res. 2005;15 1831-1837

Kerstin Lindblad-Toh, Claire M Wade, Tarjei S. Mikkelsen, Elinor K. Karlsson, David B. Jaffe, Michael Kamal, Michele Clamp, Jean L. Chang, Edward J. Kulbokas, III, Michael C. Zody, Evan Mauceli, Xiaohui Xie, Matthew Breen, Robert K. Wayne, Elaine A. Ostrander, Chris P. Ponting, Francis Galibert, Douglas R. Smith, Pieter J. deJong, Ewen Kirkness, Pablo Alvarez, Tara Biagi, William Brockman, Jonathan Butler, Chee-Wye Chin, April Cook, James Cuff, Mark J. Daly, David DeCaprio, Sante Gnerre, Manfred Grabherr, Manolis Kellis, Michael Kleber, Carolyne Bardeleben, Leo Goodstadt, Andreas Heger, Christophe Hitte, Lisa Kim, Klaus-Peter Koepfli, Heidi G. Parker, John P. Pollinger, Stephen M. J. Searle, Nathan B. Sutter, Rachael Thomas and Caleb Webber, Broad Sequencing Platform members and Eric S. Lander . Genome sequence, comparative analysis and haplotype structure of the domestic dog, Nature 438, pp 803-819 - 8 December 2005.

Jaime F. Modiano, Matthew Breen, Robert C. Burnett, Heidi G. Parker, Seidu Inusah, Rachael Thomas, Paul R. Avery, Kerstin Lindblad-Toh, Elaine A. Ostrander, Gary C. Cutter, and Anne C. Avery. Distinct B-Cell and T-Cell Lymphoproliferative Disease Prevalence among Dog Breeds Indicates Heritable Risk, Cancer Research 65: 5654-5661 - July 1st 2005. [PubMed]

Thomson, S.A., Kennerly, E., Olby, N., Mickelson, J.R., Hoffmann, D.E., Dickinson, P.J., Gibson, G., Breen. M. (2005) Microarray analysis of differentially expressed genes of primary tumors in the canine central nervous system. J. Vet. Path. (in press).

Kennerly, E., Thomson, S, Olby, N., Breen, M., Gibson, G. Comparison of regional gene expression differences ion the brains of the domestic dog and human. Human Genomics 2004 Nov;1(6):435-43. [PubMed]

Breen, M ., Hitte, C., Lorentzen, T., Thomas, R., Cadieu, E., Sabacan, L., Scott, A., Evanno, G., Parker, H.G., Kirkness, E., Hudson, R., Guyon, R., Mahairas, G.G., Gelfenbeybn, B., Fraser, C.M., André, C., Galibert, F., Ostrander, E.A. (2004). An Integrated 4300 Marker FISH/RH Map of the Canine Genome. BMC Genomics 5:65, 1-11 [PubMed]

Fosmire, S.P., Dickerson, E.B., Scott, A., Bianco, S.R., Pettengill, M., Meylemans, H., Padilla, M., Frazer-Abel, A.A., Akhtar, N., Getzy, D.M., Wojcieszyn, J., Breen, M., Helfand, S.C., Modiano, J.F. (2004). Canine Malignant Hemangiosarcoma as a Model of Primitive Angiogenic Endothelium. Lab Invest. 84, 562-572. [PubMed]

Akhtar, N, Padilla, M.L., Dickerson, E.B., Steinberg, H., Breen, M., Auerbach, R., Helfand, S.C. (2004). Interleuikin-12 inhibits tumor growth in a novel angiogenesis canine hemangiosarcom xenograft model. Neoplasia 6, 106-116 [PubMed]

Comstock, K.E., Lingaas, F., Kirkness E.F, Hitte, C., Thomas, R., Breen, M., Moe, L, Galibert, F., Ostrander, E.A. (2004). A High Resolution Map of Canine Chromosome 5q14.3-33 Spanning Human Chromosome 17p11.2 Utilizing the 1.5 x Canine Genome Sequence. Mammalian Genome 15:544-551. [PubMed]

Fosmire, S.P., Dickerson, E.B., Scott, A., Bianco, S.R., Pettengill, M., Meylemans, H., Padilla, M., Frazer-Abel, A.A., Akhtar, N., Getzy, D.M., Wojcieszyn, J., Breen, M., Helfand, S.C., Modiano, J.F. (2004). Canine Malignant Hemangiosarcoma as a Model of Primitive Angiogenic Endothelium. Lab Invest. 84, 562-572. [PubMed]

Akhtar, N, Padilla, M.L., Dickerson, E.B., Steinberg, H., Breen, M., Auerbach, R., Helfand, S.C. (2004). Interleuikin-12 inhibits tumor growth in a novel angiogenesis canine hemangiosarcom xenograft model. Neoplasia 6, 106-116 [PubMed]

Thomas, R., Fiegler, H., Ostrander, E.A., Galibert, F., Carter, N.P., Breen, M. (2003). A canine cancer gene microarray for CGH analysis of canine tumors. Cytogenetic and Genome Research 102:254-260. [PubMed]

Thomas, R, Bridge, W., Benke, K., Breen, M. (2003). Isolation, characterization and chromosomal localization of 25 cancer related canine genes. Cytogenetic and Genome Research 102:249-253. [PubMed]

Olby, N., Blot, S., Thibaud, J.L., Phillips, J.,Burr, J., Berg, J., Brown, T., Breen, M. Cerebellar cortical degeneration in adult American Staffordshire terriers. American Journal Veterinary Internal Medicine 18, 201-208. [PubMed]

Rak, S.G., Drogemuller, C., Leeb, T., Quignon,P., Andre, C., Scott, A, Breen, M., Distl. O. (2003) Cloning and chromosomal assignment of 20 candidate genes for canine congenital sensorineural deafness by FISH and RH mapping. Cytogenetic and Genome Research 101:130-135. [PubMed]

Lingaas, F., Comstock, K.E., Kirkness E.F., Sorensen, A., Aarskaug, T., Hitte, C., Nickerson, M,L, Moe, L, Schmidt, L.S., Thomas, R., Breen, M., Galibert, F., Zbar, B., Ostrander, E.A. A Mutation in the Canine BHD Gene is Associated with Hereditary Multifocal Renal Cystadenocarcinoma and Nodular Dermatofibrosis in the German Shepherd Dog. Human Molecular Genetics 12:3043-3053. [PubMed]

Langford, C.F. and Breen, M. (2003). Chromosome Structure and Function - Comparative Cytogenetics. In: Nature Encyclopedia of the Human Genome. Macmillan Publishers Ltd. Nature Publishing Group, Hampshire, U.K. www.ehgonline.net

Tiret, L., Blot, S., Kessler, J.L., Gaillot, H., Breen, M., Panthier, J.J. (2003). Mapping of the cnm locus in Labrador retrievers, a canine homologue of the human autosomal forms of the centronuclear myopathy. Human Genetics 113:297-306. [PubMed]

Thomas, R., Smith, K.C., Ostrander, E.A., Galibert, F., Breen, M. (2003). Chromosome Aberrations in Canine Multicentric Lymphomas Detected with Comparative Genomic Hybridisation and a Panel of Single Locus Probes. British Journal of Cancer 89:1530-1537. [PubMed]

Spriggs, H.F., Holmes, N.G., Breen, M., Deloukas, P., Langford, C.F., Ross, M.T., Carter, N.P., Davis, M.E., Knights, C.E., Smith, A.E., Farr. C.J., McCarthy, L.C., Binns, M.M. (2003). Construction and integration of radiation-hybrid and cytogenetic maps of dog Chromosome X. Mammalian Genome 14(3):214-221. [PubMed]

Breen, M. (2002). Equine clinical cytogenetics - human chromosomes sitting on horse chromosomes. (Invited Leader) Equine Veterinary Journal 34:110-111. [PubMed]

Breen, M., Switonski, M., Binns, M.M. (2001). Cytogenetics and Physical Chromosome Maps: Chapter 11 in Genetics of the Dog. Ruvinsky and Sampson- eds. CAB International, Oxon, U.K.

Breen, M., Jouquand S., Renier C., Mellersh C.S., Hitte C., Holmes N.G. , Cheron A., Suter N., Vignaux F., Bristow A.E., Priat C ., McCann E., Andre C., Boundy S., Gitsham P., Thomas R., Bridge W.L., Spriggs H.F., Ryder E.J., Curson A., Sampson J., Ostrander E.A., Binns M.M., and Galibert F. (2001). Chromosome-specific single locus FISH probes allow anchorage of an 1,800 marker integrated radiation-hybrid/ linkage map of the domestic dog (Canis familiaris) genome to all chromosomes. Genome Research 11:1784-1796. [PubMed]

Thomas, R., Smith, K.C., Gould, R., Gower, S.M., Binns, M.M., Breen, M. (2001). Molecular cytogenetic analysis of a novel high-grade T-lymphoblastic lymphoma demonstrating co-expression of CD3 and CD79a cell markers. Chromosome Research 8: 649-657. [PubMed]

Thomas, R., Breen, M., Deloukas, P., Holmes, N.G. and Binns, M.M (2001). An integrated physical, comparative and radiation-hybrid map of dog chromosome five. Mammalian Genome 12: 371-375. [PubMed]

Dunn, K.A., Thomas, R., Binns, M.M., Breen, M. (2000). Comparative Genomic Hybridisation (CGH) in Dogs - Application to the study of a Canine Glial Tumour Cell Line. The Veterinary Journal 160(1): 77-82. [PubMed]

Leeb, T., Neumann, S., Deppe, A., Breen, M., Brenig, B. (2000). Genomic organization of the dog dystroglycan gene (DAG) locus on chromosome 20q15.1-q15.2. Genome Research 10:295-301. [PubMed]

Binns, M.M., Swinburne, J.E., Breen, M. (2000). Molecular Genetics. Chapter 6 in 'Horse Genetics' , CAB International, Oxon, U.K. -ed A.T. Bowling.

Thomas, R., Breen, M., Langford, C.F., and Binns, M.M. (1999). Zoo-FISH analysis of dog chromosome 5: identification of conserved synteny with human and cat chromosomes. Cytogenetics and Cell Genetics 87:4-10. [PubMed]

Breen, M., Thomas, R., Binns, M.M., Carter, N.P. and Langford, C.F (1999). Reciprocal chromosome painting reveals detailed regions of synteny between the karyotypes of the domestic dog (Canis familiaris) and human. Genomics 61:145-155. [PubMed]

Breen, M., Bullerdiek, J and Langford, C. (1999). The DAPI banded karyotype of the domestic dog (Canis familiaris) generated using chromosome specific paint probes. Chromosome Research 7:401-406 [+erratum vol 7:575] [PubMed]

Schatzberg, S.J., Olby, N.J., Breen, M., Anderson, L.V.B., Langford, C.F., Dickens, H.F., Wilton, S.D., Zeiss, C.J., Binns, M.M., Kornegay, J.N.,Morris, G., Sharp, N.J.H. (1999). Molecular analysis of a spontaneous dystrophin 'knockout' dog. Neuromuscular Disorders 9:289-295. [PubMed]

van der Sluis, B.J.A., Breen, M., Nanji, M., van Wolferen, M., de Jong, P., Binns, M.M., Pearson, P.L., Kuipers, J., Rothuizen, J., Cox, D.W.,Wijmenga, C., van Oost, B. (1999). Genetic mapping of the copper toxicosis locus in Bedlington terriers to dog chromosome 10, in a region syntenic to human chromosome region 2p13-16. Human Molecular Genetics 8:501-507. [PubMed]

Breen, M., Langford, C.F., Carter, N.P., Holmes, N.G., Dickens, H.F., Thomas, R., Suter, N., Ryder, E.J., Pope, M. and Binns, M.M. (1999). FISH mapping and identification of canine chromosomes. Journal of Heredity 90:27-30. [PubMed]

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