Ghashghaei, Troy, Ph.D. (PI)
Associate Professor of Neurobiology
Ph.D.: Boston University
Postdoctoral: University of North Carolina at Chapel Hill
PI's Office Phone: (919) 513-6174
Fax: (919) 513-6465
Projects in our laboratory are focused on developmental neurobiology:
1. Development and aging of the adult stem cells and their ependymal niche in the forebrain. We use mouse genetics in combination with molecular, biochemical, and cell biological approaches to address fundamental questions regarding the functional significance of ependymal cells during development and aging.
Current projects are investigating:
a. Mechanisms driving differentiation of ependymal cells and their responses to various stimuli.
b. Mechanisms that help maintain ependymal homeostasis during aging.
2. Role of cell cycle regulators in symmetric and asymmetric divisions of neural stem cells in the developing and postnatal brain. We use mouse genetics, biochemical assays, and state-of-the-art imaging tools to understand mechanisms that regulate the decision of neural stem cells to divide symmetrically or asymmetrical in the embryonic and postnatal stem cell niches.
Current Projects are investigating:
a. Symmetric versus asymmetric decisions in dividing stem cells.
b. Cell-cell communication among clones of progenitors and their role in neuronal and glial fate specification.
Highly motivated postdoctoral fellows and graduate students interested in our research should contact Dr. Ghashghaei (Troy_Ghashghaei@ncsu.edu)
Xuying Zhang (Lab Manager)
|Contact Information: 1060 William Moore Dr., CVM Research building, RB230 and RB236, Raleigh, NC 27607|
|Staff Office Phone: (919) 513-0955||Lab1 Phone: (919) 513-6840||Lab2 Phone: (919) 513-6841|
Murlidharan G, Corriher T, Ghashghaei H.T., Asokan A. (2015) Unique glycan signatures regulate adeno-associated viral tropism in the developing brain. J Virol. pii: JVI.02951-14. [Epub ahead of print]
Hammad M., Schmidt S.L., Zhang X., Bray R., Frohlich F., Ghashghaei H.T. (2014) Transplantation of GABAergic Interneurons into the Neonatal Primary Visual Cortex Reduces Absence Seizures in Stargazer Mice. Cereb Cortex. [Epub ahead of print]
Muthusamy N., Vijayakumar A., Cheng J., Ghashghaei H.T. (2014) A Knock-in Foxj1CreERT2::GFP mouse for recombination in epithelial cells with motile cilia. Genesis. 52(4):350-8. "Cover Illustration"
Liang H., Xiao G., Yin H., Hippenmeyer S., Horowitz J.M., Ghashghaei H.T. (2013) Neural development is dependent on the function of specificity protein 2 in cell cycle progression. Development. 140(3):552-61.
Nehrenberg D.L., Sheikh A., Ghashghaei H.T. (2013) Identification of neuronal loci involved with displays of affective aggression in NC900 mice. Brain Struct. Funct. 218(4):1033-49.
Liang H., Hippenmeyer S., Ghashghaei H.T. (2012) A Nestin-cre transgenic mouse isinsufficient for recombination in early embryonic neural progenitors. Biol Open. 1(12):1200-3. "Cover Illustration"
Jacquet B.V., Muthusamy N., Sommerville L.J., Xiao G., Liang H., Zhang Y., Holtzman M.J., Ghashghaei H.T. (2011) Specification of a Foxj1-dependent lineage in the forebrain is required for embryonic-to-postnatal transition of neurogenesis in the olfactory bulb. Journal of Neuroscience. 31(25):9368-82.
Jacquet B.V., Ruckart P., Ghashghaei H.T. (2010) An organotypic slice assay for high-resolution time-lapse imaging of neuronal migration in the postnatal brain. J Vis Exp. (46). pii: 2486. doi: 10.3791/2486.
Jacquet B.V.,Salinas-Mondragon R., Liang H., Therit B., Buie J.D., Dykstra M., Campbell K., Ostrowski L.E., Brody S.L.,and Ghashghaei H.T. (2009) FoxJ1-dependent gene expression is required for differentiation of radial glia into ependymal cells and a subset of astrocytes in the postnatal brain. Development. 136, 4021-4031. "Featured article with Cover Illustration".
Jacquet B.V., Patel M., Iyengar M., Liang H., Therit B., Salinas-Mondragon R., Lai C., Olsen J.C., Anton E.S., Ghashghaei H.T. (2009) Analysis of neuronal proliferation, migration and differentiation in the postnatal brain using equine infectious anemia virus-based lentiviral vectors. Gene Therapy. 16(8):1021-33.
Ghashghaei H.T., Weimer J.M., Schmid R.S., Yokota Y., McCarthy K.D., Popko B., Anton E.S. (2007) Reinduction of ErbB2 in astrocytes promotes radial glial progenitor identity in adult cerebral cortex. Genes Dev. 21(24): 3258-71.
Yokota, Y., Ghashghaei, H.T., Han, C., Watson, H., Campbell, K.J., Anton, E.S., (2007) Radial glial dependent and independent dynamics of interneuronal migration in the developing cerebral cortex. PLoS ONE. 2(8): e794.
Ghashghaei H.T., Lai C., and Anton E.S. (2007) Neuronal migration in the adult brain: are we there yet? Nature Reviews Neuroscience. 8(2):141-151.
Ghashghaei H.T., Weber J., Pevny L., Schmid R., Schwab M.H., Lloyd K.C., Eisenstat D.D., Lai C., Anton E.S. (2006) The role of neuregulin-ErbB4 interactions on the proliferation and organization of cells in the subventricular zone. Proceeding of the National Academy of Sciences103(6): 1930-1935.
Anton E.S.*, Ghashghaei H.T.*, Weber J.L., McCann C., Fischer T.M., Cheung I.D., Gassmann M., Messing A., Klein R., Schwab M.H., Lloyd K.C.K., Lai C. (2004) Receptor tyrosine kinase ErbB4 modulates neuroblast migration and placement in the adult forebrain. Nature Neuroscience 7(12): 1319-1328. *Authors contributed equally