Hudson, Lola, DVM, PhD
Professor, College of Veterinary Medicine, Department of Molecular Biomedical Sciences (MBS)
D.V.M.: University of Tennessee, Knoxville, TN
Ph.D.: Cornell University, Ithaca, NY
Phone: (919) 513-6306
Fax: (919) 514-6465
The goal of research in this laboratory is the study of viral neuropathogenesis and mechanisms of virus entry into the CNS. We have investigated in vivo rabies and feline immunodeficiency virus (FIV), which have widely divergent mechanisms of CNS entry. The laboratory has developed an in vitro model using feline cells to determine the conditions under which immune cells in normal and FIV-infected animals are capable of penetrating the brain endothelium, mechanism of attachment to the endothelium such as up-regulation of specific adhesion molecules, and characteristics of attaching cells.
My laboratory has developed an in vitro brain endothelium using purified or enriched primary cultures of endothelial cells and astrocytes on opposite sides of porous well inserts. This allows us the luxury of adding further factors and cell types to the lower chamber to study the transmigration of peripheral blood mononuclear cells under normal and inflammatory conditions. This model is adaptable to cells from different species- we are now using both feline and murine models.
Additionally, we are studying neuropathogenesis of feline immunodeficiency virus (FIV) as an animal model of HIV. This includes studies on the transport of virus across the endothelium and the role of the CNS as a reservoir able to re-seed peripheral tissues after retroviral therapy and inability to detect viremia.
Equipment and Techniques:
Much of our research requires purified primary cell cultures of brain capillary endothelial cells, astrocytes, microglia and dissociated neuronal-glial mixed cell cultures. Therefore we have all the basic equipment for performing the isolations and passaging/freezing cultures including CO2 incubators, laminar flow hood, and inverted phase contrast and fluorescent microscope.
Through various service laboratories, we can perform needed clinical pathology tests such as complete blood counts, flow cytometry, cell sorting, confocal microscopy, and electron microscopy. Through one of our collaborators, we have access to advanced morphometrics software allowing densitometric studies, and rapid cell counting procedures.
We also have expertise in immunohistochemistry and immunocytochemistry of frozen and formalin-fixed tissues and cells. This includes immunofluorescence, and double-labeling techniques. As a related technique, we perform Western Blot analysis. As the antibodies of interest are rarely known to be effective on feline cells, our protocols include testing for cross-specificity.
To include mechanistic investigations of cytokine and adhesion molecule function at the endothelium, other molecular biology our laboratory is performing techniques such as RNA extraction and purification, RT-PCR, and heteroduplex tracking assay (HTA) .
LC Hudson , DC Bragg, MB Tompkins, RB Meeker. Astrocytes and microglia differentially regulate trafficking of lymphocyte subsets across brain endothelial cells under uninfected and infected conditions. Brain Research (submitted)
LC Hudson , Y-H Liang, JS Yang, MB Tompkins. CNS Invasion During Acute Feline Immunodeficiency Virus Infection Is Not Detected By Magnetic Resonance Spectroscopy and Brain Stem Auditory Evoked Response. Am J Vet Res (submitted)
LC Hudson , TW Vahlenkamp, KE Howard, B Colby, MB Tompkins, RB Meeker. Cerebrospinal fluid centesis at the cerebellomedullary cistern of kittens. Contemporary Topics 41(2002): 30-32.
DC Bragg, LC Hudson, Y-H Liang, MB Tompkins, A Fernandes, RB Meeker. Proliferation of choroid plexus macrophages in response to FIV. J of NeuroVirology 8 (2002):225-239
Love, N, Fisher, P, Hudson, LC. The computed tomographic (CT) enhancement pattern of the normal canine pituitary gland. Vet Radiology & Ultrasound 41(2000): 507-510.
Hudson, LC , Berschneider, HM, Ferris, KK, Vivrette, SL. Disaster relief management of companion animals affected by the floods of Hurricane Floyd J Am Vet Med Ass 218(2001):354-359.
Liang,Y-H, Hudson, LC, English, RV, Levy, JK, Ritchey, JW, Tompkins, WAF, Tompkins, MB. The effects of FIV infection on early thymocyte differentiation and cytokine production in the thymus. J of Infectious Diseases 181(2000):564-575.
Jordan, H L, Liang, Y, Hudson, LC, Tompkins, WA. Expression of feline immunodeficiency virus in semen during acute infection. Am J Vet Res 60 (1999): 211-215.
NE Love, P Fisher, LC Hudson. The computed tomographic (CT) enhancement pattern of the normal canine pituitary gland. Proceeding, ACVR, Banff, August 1998.
Hudson, LC, Bragg, DC, Meeker, RB. Development of an in vitro blood brain barrier to study FIV neuropathogenesis. Proceedings, Fourth International Feline Retrovirus Research Symposium. Glasgow, Scotland. 1998.
Hughes, CS, SL Vaden, CA Manaugh, GS Price, LC Hudson. Modulation of doxorubicin concentration by cyclosporin A in barrier tissues expressing P-glycoprotein in rats J of Neuro-Oncology 37:45-54 (1998).
MB Tompkins, Department of MPP, College of Veterinary Medicine, North Carolina State University
RB Meeker, Department of Neurology, College of Medicine, University of North Carolina at Chapel Hill
IW Smoak, Department of MBS, College of Veterinary Medicine, North Carolina State University
LD Martin, Department of MBS, College of Veterinary Medicine, North Carolina State University
Colby, Brenda, PhD