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Piedrahita, Jorge

Jorge Piedrahita

 

Professor of Genomics
Dept. of Molecular Biomedical Sciences

E-mail: jorge_piedrahita@ncsu.edu


 

Current Research:

Jorge Piedrahita’s laboratory is primarily interested in the understanding of the role of imprinted genes in embryo development and in disease, and the development of transgenic animals of use in human and veterinary medicine, and in agriculture. Towards this end, we combine techniques in functional genomics, cell biology, embryo manipulation, and molecular biology. Specifically, our efforts concentrate in the use of gene expression profiles in models of imprinting disregulation and in functional genomic analysis of candidate genes. Presently, our research is focused on: a) The identification and functional analysis of imprinted genes in humans and swine. b) The characterization of embryonic stem cell lines and induced pluripotential stem cells from a variety of mammals. c) The development of porcine models of human disease using somatic cell nuclear transfer.

Representative Publications:

Piedrahita JA , Zhang SH, Hagaman JR, Clark PM, and Maeda N. 1992. Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in mouse embryonic stem cells. Proc. Natl. Acad. Sci. USA 89:4471-4475.

Piedrahita JA , Gillespie L, and Maeda N. 1992. Production of chimeric hamsters by aggregation of eight-cell embryos. Biology of Reproduction 47:347-354.

Zhang SH, Reddick RL, Piedrahita JA, and Maeda N. 1992. Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein E. Science 258:468-471.

Rucker E, and Piedrahita JA. 1997. Cre-mediated site-directed insertions at the mouse whey acidic protein (mWAP) locus. Mol. Repr. Devel. 48: 324-331.

Vazquez JC, Nogues C, Rucker E, and Piedrahita JA. 1998. Factors affecting the efficiency of introducing small mutation in specific genes by homologous recombination; Cre-loxP versus the tag-and-exchange. Transgenic Research 7, 181-193.

Piedrahita JA , Oetama B, Bennett G, Waes JV, Lacey SW, Kamen B, Richardson J, Lark R, and Finnell R. 1999. Inactivation of the folate binding protein genes disrupts neural tube closure. Nature Genetics, 23:228-232.

Piedrahita JA , Moore K, Oetama B, Lee C-K, Scales N, Ramsoondar J, Bazer F, and Ott T. 1998. Generation of transgenic porcine chimeras using primordial germ cell (PGC)-derived colonies. Biol. Reprod. 58, 1321-1329.

Ramsoondar JJ, Christopherson RJ, Guilbert LJ, Dixon WT, Ghahary A, Ellis S, Wegmann TG, Piedrahita JA. 1999. Regulated Expression of MHC class I molecules in the porcine embryo. Biol. Reprod. 60:387-397.

Lee C-K, Weaks R, Johnson G, Bazer F , and Piedrahita, JA 2000. Effects of protease inhibitors and antioxidants on in vitro survival of porcine primordial germ cells. Biol. Reprod. 63:887-897.

Lee C-K, and Piedrahita JA. 2000. Effects of growth factors and feeder cells on porcine primordial germ cells in vitro. Cloning. 2:197-206.

Westhusin M, and Piedrahita JA. 2000. Three little pigs worth the huff and puff?. Nature Biotechnol. 18:1144-1145.

Piedrahita JA . 2000. Gene targeting in domestic species: A new beginning. Transgenic Res. 9:261-262.

Piedrahita JA , Wells DN, Miller AL, Oliver JE , Berg MC , Peterson AJ, Tervit RH. 2002. Effects of Follicular Size of Cytoplast Donor on the Efficiency of Cloning in Cattle. Mol. Reprod. Dev. 61:317-326.

Lee CK, Piedrahita JA. 2002. Inhibition of apoptosis in serum starved porcine embryonic fibroblasts. Mol. Reprod. Devel. 62:106-112.

Walker SC, Shin TY, Zaunbrecher GM, Romano JE, Johnson, GA, Bazer, FW and Piedrahita JA. 2002. A Highly Efficient Method for Porcine Cloning by Nuclear Transfer Using In Vitro-Matured Oocytes. Cloning Stem Cells 4:105-112.

Archer G, Friend, T, Piedrahita JA, Neville C, Walker SC. 2003. Behavioral Variation in Cloned Pigs. Applied Animal Behavior 2012:1-11.

Kim H-T, Song I-Y, Piedrahita JA . 2003. Kidney-specific activity of bovine uromodulin promoter in transgenic mice. Transgenic Research 12:191-201.

Mir B, Tanner N, Chowdhary BP, Piedrahita JA. 2003. UP1 Extends Life of Primary Porcine FetalFibroblasts in Culture. Cloning and Stem Cells 5: 143-148.

Archer GS, Dindot S, Friend TH, Walker S, Zaunbrecher G, Lawhorn B, Piedrahita JA. 2003. Hierarchical Phenotypic and Epigenetic Variation in Cloned Swine. Biol. Reprod. 69:430-436.

Thomson J, Rucker E, and Piedrahita JA . 2003. Mutational analysis of loxP sites for efficient cre-mediated insertion into genomic DNA. Genesis 36:162-167.

Piedrahita JA, Mir B, Dindot S, and Walker S. 2004. Somatic cell cloning: The ultimate form of nuclear reprogramming?. J Amer. Soc. Nephrol. 15:1140-1144.

Farin C, Farin PW, and Piedrahita JA . 2004. Development of fetuses from in vitro produced and cloned bovine embryos. J. Animal Sc. 82:53-62

Dindot S, Farin C, Farin P, Miles J, Walker S, Long C, and Piedrahita JA . 2004. Epigenetic and genomic imprinting analysis in nuclear transfer derived Bos gaurus/Bos taurus hybrid fetuses. Biol. Reprod. 71:470-478.

Mir B and Piedrahita JA . 2004. Nuclear localization signal and cell synchrony enhances homologous recombination in fetal fibroblasts. Nucleic Acid Res. 11:e25-e28.

Piedrahita JA and Mir B. 2004. Cloning and Transgenesis in Mammals; Implications for Xenotransplantation. Am. J. Transpl. 6:43-50.

Benjamin CL, Jenster G, and Piedrahita JA. 2004. Use of artificial androgen receptor coactivators to alter myoblast proliferation. J. Steriod Bioch. Mol. Biol. 91:111-119..

Dindot S, Kent KC, Evers B, Loskutoff N, Womack J and Piedrahita JA. 2004. Conservation of genomic imprinting at the XIST, IGF2, and GTL2 loci in the bovine. Mammalian Genome 15:828-833.

Mir B, Zaunbrecher G, Archer GS, Friend T, and Piedrahita JA. 2005. Progeny of somatic cell nuclear transfer (SCNT) clones of phenotypically similar to non-cloned pigs. Cloning and Stem Cells. 7:119-125.

Tsai S, Cassady JP, Freking BA, Nonneman DJ, Rohrer GA, Piedrahita JA. 2006. Annotation of the Affymetrix porcine genome microarray. Anim Genet. 37:423-4.

Monteiro-Riviere N, Inman A, Hedgpeth V, Mosteller B, Piedrahita J. 2006. Dermatological effects of chronic exposure to 7,12-dimethylbenz[Ajanthracene(DMBA) or N-methyl-N-nitrosoguanidine (MNNG) in swine. Cutan Ocul Toxicol 25:103-19.

Farin PW, Piedrahita JA, Farin CE. 2006. Errors in development of fetuses and placentas from in vitro-produced bovine embryos. Theriogenology 65:178-91.

Monteiro_Riviere N, Hedgepeth N, Inman A, Mosteller B, and Piedrahita JA. 2006. Dermatological effects of chronic exposure to 7,12-dimethylbenz[a]anthracene (DMBA) of n-methyl-n-nitrosoguanidine (MNNG) in swine. J Cutanoeus Ocular Tox 25:103-119.

Tsai S, Cassady J, Freking D, Nonneman G. Rohrer G, Piedrahita JA. 2006. Annotation of Affymetrix Porcine genome Microarray Chip. Animal Genetics 37:423-424.

Tsai S, Mir B, Martin AC, Estrada JL, Bischoff SR, Hsieh WP, Cassady JP, Freking BA, Nonneman DJ, Rohrer GA, Piedrahita JA. 2006. Detection of transcriptional difference of porcine imprinted genes using different microarray platforms. BMC Genomics. 7:328-336.

Estrada J, Sommer J, Collins B, Mir B, Martin A, York A, Petters RM, and Piedrahita JA 2007. Swine generated by somatic cell nuclear transfer have increased incidence of intra uterine growth restriction (IUGR). Cloning and Stem Cells  9:229-236

Estrada J, Sommer J, Collins B, Mir B, Martin A, York A, Petters RM, Piedrahita JA. Swine generated by somatic cell nuclear transfer have increased incidence of intrauterine growth restriction (IUGR). Cloning Stem Cells. 2007 Summer;9(2):229-36.

Zaunbrecher G, Mir B, Dunne PW, Breen M, Piedrahita JA. 2008. Enhancement of extra chromosomal recombination in somatic cells by affecting the ratio of homologous recombination (HR) to non-homologous end joining (NHEJ). Animal Biotechnology. 19:6-21.

Lee E, Estrada JL, Piedrahita JA. 2008. A comparative study on the efficiency of two enucleation methods in pig somatic cell nuclear transfer: effects of the squeezing and the aspiration methods. Animal Biotechnology. 19:71-79.

Estrada JL, Collins B, York A, Bischoff SR, Sommer J, Tsai S, Petters RM, Piedrahita JA. 2008. Successful cloning of the Yucatan minipig using commercial/occidental breeds as oocyte donors and embryo recipients. Cloning and Stem Cells. 10:287-296

Bischoff SR, Tsai, S, Hardison NE, York AM, Freking BA, Nonneman D, Rohrer G, Piedrahita JA. 2008. Identification of SNPs and INDELS in swine using short oligonucleotide microarrays. BMC Genomics. 9:252-263.

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Dr. Piedrahita's lab personnel