Nikon C1
The fully digital Nikon C1 confocal laser scanning system was installed in 2003. It is attached to a motorized Nikon Eclipse 2000E inverted light microscope. The microscope has bright field, phase contrast, differential interference contrast (DIC), and polarizing optical capabilities. It is equipped with a broad variety of epifluorescence filter cubes and a digital camera. The confocal system is supplied with 3 lasers (red HeNe, green HeNe, and Argon) and can also import DIC images generated by the mercury arc light source. The stage is fully automated and can produce z-stacks through a specimen and assemble them into 3-D volumes. A heated stage is available for suitable studies. Some of the different sample types recorded by this instrument are shown below:
Plant Root (Arabidopsis).
Single Frame from a Movie clip showing cultured cells with DIC imaging and a scanning laser produced localization (red) of transferrin taken up by the cells from the culture medium.
Single Frame from a Movie clip showing a laser scanning image of fluorochrome-labeled (green) neural cells in a brain slice.