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Sample Preparation

 

FEI/Philips EM 208S

The FEI/Philips EM 208S transmission electron microscope, installed in 1999, captures images on 3.25 X4" film that is developed and printed in our darkroom. In December 2008, a Gatan 785-ES 1000W Digital Camera was attached to the microscope for producing digital images. Some of the different sample types recorded by this instrument are shown below:

Borrelia burgdorferi

Borrelia burgdorferi:
A specific antibody raised against a flagellar protein (the primary antibody) was incubated with living cells. Next the bacterial cells were incubated with a gold-labeled antibody against the primary antibody (the secondary antibody). Finally the bacterial cells were negative-stained with 2% phosphotungsic acid (PTA) to provide contrast. The bacterial flagella are covered with the gold label attached to the secondary antibody (the small black dots).

Lymphocystis iridis

Lymphocystis iridis
is an iridovirus that causes skin infections in fish. The dark polyhedral structures in this skin cell are viral particles.

Drosophila melanogaster

Drosophila melanogaster (fruit fly) spermatids showing numerous flagellar shafts in cross section with typical 9+2 microtubular arrays and dark amorphous acrosomal material.
Image colorized in Adobe PhotoShop.

Rat Kidney

Rat Kidney,
perfused with 4F:1G , showing cells of a proximal convoluted tubule.

Rat Kidney

Rat Kidney,
perfused with 4F:1G , showing cells of a glomerulus.